NASC

NASC ID: N849176

Name: WiscDsLox6H08

ABRC stock number: CS849176

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

Wiscseq_DsLox6H08.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT1G29750.1 (at chr 1 pos 10414010 (C/10414010-10414097) on the TAIR10). The sequences' 1-87 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 0, R 1, C 8 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO452555.

Wiscseq_DsLox6H08.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT5G50420.1 (chr 5 pos 20532792 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG810105.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox6H08

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox6H08	WiscDsLox6H08	AT1G29750 AT5G50420 At1g32320